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寡霉素抑制离体玉米根对磷酸盐的摄取和 ATP 的标记。

Oligomycin inhibition of phosphate uptake and ATP labeling in excised maize roots.

机构信息

Phosphorus Laboratory, Soil and Water Conservation Research Division, ARS-USDA and Colorado State University, Fort Collins, Colorado 80521.

出版信息

Plant Physiol. 1969 Jul;44(7):1040-4. doi: 10.1104/pp.44.7.1040.

DOI:10.1104/pp.44.7.1040
PMID:16657154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC396211/
Abstract

ATP labeling by newly absorbed (32)P in excised maize roots was reduced 34% by the presence of oligomycin during a 4-min uptake period with no reduction in rate of phosphorus absorption. Longer exposure to oligomycin, during pretreatment periods or longer uptake periods, reduced phosphorus absorption and further reduced ATP synthesis. In these tissues it appears that oligomycin inhibits ATP production at the mitochondria, that ATP is the energy source for phosphorus uptake at the plasmalemma, and that a depletion in the ATP supply causes a reduced rate of uptake.

摘要

用新吸收的(32)P 对离体玉米根进行 ATP 标记,在 4 分钟的吸收期内,寡霉素的存在使标记的 ATP 减少了 34%,而磷的吸收速率没有降低。寡霉素在预处理期或较长的吸收期内较长时间存在,会降低磷的吸收,并进一步降低 ATP 的合成。在这些组织中,寡霉素似乎抑制了线粒体中的 ATP 生成,ATP 是质膜磷吸收的能量来源,而 ATP 供应的枯竭导致吸收速率降低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55db/396211/2aa51d36e329/plntphys00212-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55db/396211/2aa51d36e329/plntphys00212-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55db/396211/2aa51d36e329/plntphys00212-0105-a.jpg

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本文引用的文献

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Plant Physiol. 1967 Aug;42(8):1103-10. doi: 10.1104/pp.42.8.1103.
2
Phosphorylation by Barley Root Mitochondria & Phosphate Absorption by Barley Roots.大麦根线粒体的磷酸化作用与大麦根对磷酸盐的吸收
Plant Physiol. 1962 Jan;37(1):8-17. doi: 10.1104/pp.37.1.8.
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Absorption of Ionic Species of Orthophosphate by Barley Roots: Effects of 2,4-Dinitrophenol and Oxygen Tension.
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