Department of Botany, Rutgers University, New Brunswick, New Jersey 08903.
Plant Physiol. 1969 Jul;44(7):1073-9. doi: 10.1104/pp.44.7.1073.
Explants of genetic tumors, tumors initiated by Agrobacterium tumefaciens strains B-6 and T-37, and excised pith plugs from Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii were cultured on Murashige and Skoog medium. All cultures, pith callus and tumors with the exception of N. langsdorffii pith grew on this medium. Addition of glutamine to the medium resulted in highly organoid growth in N. langsdorffii pith. In order to have material comparable to other pith cultures, N. langsdorffii was initiated on 2,4-dichlorophenoxyacetic acid medium, after which it grows on complete medium as amorphous pith callus. Except for the initiation of N. langsdorffii (and N. glauca) pith, the 2,4-dichlorophenoxyacetic acid medium, caused bleaching in cultures of T-37 induced tumors and death of B-6 induced tumors. Tumor cultures, except for the seedling tumor, grew well on a minimal medium lacking kinetin, indoleacetic acid, vitamins, glycine, and inositol. Glycine was necessary only in the growth of N. langsdorffii pith callus. A tissue culture model is presented which permits comparison of the various tissue types.
遗传肿瘤外植体、根癌农杆菌菌株 B-6 和 T-37 引发的肿瘤，以及Nicotiana glauca、N. langsdorffii 和 N. glauca-langsdorffii 的髓心插条，在 Murashige 和 Skoog 培养基上进行培养。除了 N. langsdorffii 的髓心外，所有的培养物、髓心愈伤组织和肿瘤都在这种培养基上生长。在培养基中添加谷氨酰胺会导致 N. langsdorffii 髓心高度器官样生长。为了获得与其他髓心培养物可比的材料，N. langsdorffii 先在 2,4-二氯苯氧乙酸培养基上起始培养，然后在完全培养基上作为无定形髓心愈伤组织生长。除了 N. langsdorffii（和 N. glauca）髓心的起始培养外，2,4-二氯苯氧乙酸培养基会导致 T-37 诱导的肿瘤培养物白化和 B-6 诱导的肿瘤死亡。除了幼苗肿瘤外，肿瘤培养物在缺乏激动素、吲哚乙酸、维生素、甘氨酸和肌醇的最小培养基上生长良好。只有在 N. langsdorffii 髓心愈伤组织的生长中才需要甘氨酸。提出了一种组织培养模型，允许比较各种组织类型。
