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γ射线辐照激活生化系统:植物愈伤组织中硝酸还原酶活性的诱导。

Gamma-irradiation activates biochemical systems: induction of nitrate reductase activity in plant callus.

作者信息

Pandey K N, Sabharwal P S

出版信息

Proc Natl Acad Sci U S A. 1982 Sep;79(18):5460-4. doi: 10.1073/pnas.79.18.5460.

Abstract

Gamma-irradiation induced high levels of nitrate reductase activity (NADH:nitrate oxidoreductase, EC 1.6.6.1) in callus of Haworthia mirabilis Haworth. Subcultures of gamma-irradiated tissues showed autonomous growth on minimal medium. We were able to mimic the effects of gamma-irradiation by inducing nitrate reductase activity in unirradiated callus with exogenous auxin and kinetin. These results revealed that induction of nitrate reductase activity by gamma-irradiation is mediated through in vitro activation of hormone synthesis in callus cells.

摘要

伽马射线辐照诱导了十二卷属植物(Haworthia mirabilis Haworth)愈伤组织中高水平的硝酸还原酶活性(NADH:硝酸氧化还原酶,EC 1.6.6.1)。伽马射线辐照组织的继代培养物在基本培养基上表现出自主生长。我们能够通过用外源生长素和激动素诱导未辐照愈伤组织中的硝酸还原酶活性来模拟伽马射线辐照的效果。这些结果表明,伽马射线辐照对硝酸还原酶活性的诱导是通过愈伤组织细胞中激素合成的体外激活介导的。

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