大麦中存在一种在黑暗中使硝酸还原酶失活的系统的证据，该系统需要蛋白质合成。
Evidence for an Inactivating System of Nitrate Reductase in Hordeum vulgare L. during Darkness That Requires Protein Synthesis.
作者信息
Travis R L, Jordan W R, Huffaker R C
机构信息
Department of Agronomy and Range Science, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1969 Aug;44(8):1150-6. doi: 10.1104/pp.44.8.1150.
Abstract
The disappearance of nitrate reductase activity in leaves of Hordeum vulgare L. during darkness was inhibited by cycloheximide, actinomycin D, and low temperature. Thus, protein synthesis was probably required for the disappearance of nitrate reductase in the dark. Since chloramphenicol did not affect the rate of loss of activity, the degradation or inactivation apparently required protein synthesis by the cytoplasmic ribosomal system. Consistent with this observation, nitrate reductase is also reportedly located in the cytoplasm. Thus, the amount of nitrate reductase activity present in leaves of barley may be controlled by a balance between activating and inactivating systems.
摘要
大麦叶片中硝酸还原酶活性在黑暗期间的消失受到环己酰亚胺、放线菌素D和低温的抑制。因此，黑暗中硝酸还原酶的消失可能需要蛋白质合成。由于氯霉素不影响活性丧失的速率，降解或失活显然需要细胞质核糖体系统进行蛋白质合成。与这一观察结果一致，据报道硝酸还原酶也位于细胞质中。因此，大麦叶片中硝酸还原酶活性的量可能由激活和失活系统之间的平衡来控制。
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