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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Freitas T R, Romero C H

Laboratório Nacional de Referência Animal (LANARA), Ministério da Agricultura e Reforma Agrária (MARA), Pedro Leopoldo, MG, Brasil.

Braz J Med Biol Res. 1991;24(10):1017-23.

Wild stable flies (Stomoxys calcitrans) feeding on heifers infected with bovine leukosis virus (BLV) carried viable bovine leucocytes in the midgut and proboscis that, when inoculated by the subcutaneous route into lambs aged 5 to 60 days, elicited the development of antibodies to glycoprotein (gp51) and polypeptide 25 (p25). 2. Antibodies were detected as early as one month later and persisted for an experimental period of 24 or 36 months. Uninoculated control lambs reared together with the experimental animals did not acquire the infection, indicating the lack of horizontal transmission. 3. S. calcitrans reared in the laboratory were intermittently allowed to feed on the skin of BLV-infected heifers and on five lambs over a period of 3-10 months. Although some of these lambs were bitten about 500 times, none developed antibodies to BLV (gp51 or p25) over observation periods of 30 or 36 months.

以感染牛白血病病毒（BLV）的小母牛为食的野生厩螫蝇（Stomoxys calcitrans），其肠道和喙部携带存活的牛白细胞，当通过皮下途径接种到5至60日龄的羔羊体内时，会引发针对糖蛋白（gp51）和多肽25（p25）的抗体产生。2. 最早在一个月后检测到抗体，并在24或36个月的实验期内持续存在。与实验动物一起饲养的未接种对照羔羊未感染，表明不存在水平传播。3. 在实验室饲养的厩螫蝇在3至10个月的时间里，间歇性地让其取食感染BLV的小母牛的皮肤以及五只羔羊。尽管其中一些羔羊被叮咬了约500次，但在30或36个月的观察期内，没有一只产生针对BLV（gp51或p25）的抗体。

Freitas T R, Romero C H

Laboratório Nacional de Referência Animal (LANARA), Ministério da Agricultura e Reforma Agrária (MARA), Pedro Leopoldo, MG, Brasil.

Braz J Med Biol Res. 1991;24(10):1017-23.

Wild stable flies (Stomoxys calcitrans) feeding on heifers infected with bovine leukosis virus (BLV) carried viable bovine leucocytes in the midgut and proboscis that, when inoculated by the subcutaneous route into lambs aged 5 to 60 days, elicited the development of antibodies to glycoprotein (gp51) and polypeptide 25 (p25). 2. Antibodies were detected as early as one month later and persisted for an experimental period of 24 or 36 months. Uninoculated control lambs reared together with the experimental animals did not acquire the infection, indicating the lack of horizontal transmission. 3. S. calcitrans reared in the laboratory were intermittently allowed to feed on the skin of BLV-infected heifers and on five lambs over a period of 3-10 months. Although some of these lambs were bitten about 500 times, none developed antibodies to BLV (gp51 or p25) over observation periods of 30 or 36 months.

以感染牛白血病病毒（BLV）的小母牛为食的野生厩螫蝇（Stomoxys calcitrans），其肠道和喙部携带存活的牛白细胞，当通过皮下途径接种到5至60日龄的羔羊体内时，会引发针对糖蛋白（gp51）和多肽25（p25）的抗体产生。2. 最早在一个月后检测到抗体，并在24或36个月的实验期内持续存在。与实验动物一起饲养的未接种对照羔羊未感染，表明不存在水平传播。3. 在实验室饲养的厩螫蝇在3至10个月的时间里，间歇性地让其取食感染BLV的小母牛的皮肤以及五只羔羊。尽管其中一些羔羊被叮咬了约500次，但在30或36个月的观察期内，没有一只产生针对BLV（gp51或p25）的抗体。