Ratner A, Goren R, Monselise S P
Department of Citriculture, Faculty of Agriculture, Hebrew University, Rehovot, Israel.
Plant Physiol. 1969 Dec;44(12):1717-23. doi: 10.1104/pp.44.12.1717.
The activity of pectin esterase and cellulase in abscission of citrus explants was studied. No relation was established between pectin esterase and abscission, while cellulase activity was markedly increased before abscission and for a certain period after excision. IAA and cycloheximide delay abscission and cellulase activity, while ethylene and, to a lesser extent, GA(3) accelerate them. Application of cycloheximide during the lag period and before cellulase activity can be measured, inhibits to a certain extent the formation of cellulase. An escape from the inhibitory effect of cycloheximide is detected when inhibitor is supplied at the end of the lag period.
研究了柑橘外植体脱落过程中果胶酯酶和纤维素酶的活性。未发现果胶酯酶与脱落之间存在关联，而纤维素酶活性在脱落前及切除后的一段时间内显著增加。吲哚乙酸（IAA）和环己酰亚胺可延迟脱落和纤维素酶活性，而乙烯以及在较小程度上的赤霉素（GA₃）则加速它们。在延迟期且在可测量纤维素酶活性之前应用环己酰亚胺，在一定程度上抑制纤维素酶的形成。当在延迟期结束时供应抑制剂时，可检测到对环己酰亚胺抑制作用的逃避。
