Musgrave A, Kende H
Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.
Plant Physiol. 1970 Jan;45(1):56-61. doi: 10.1104/pp.45.1.56.
Radioactive gibberellin A(5) ((3)H-GA(5)) was synthesized from gibberellic acid. When it was applied to dwarf peas grown in the dark, an average of 3% was converted to another acid gibberellin within 48 hours. The biological activity of the metabolite did not account for the response to applied GA(5). GA(5) is therefore assumed to be biologically active per se.(3)H-GA(5) did not appear to form a stable complex with a macromolecule in pea shoots. When injected into dwarf pea pods, (3)H-GA(5) was readily metabolized by maturing seed to more water-soluble substances and to two other acidic compounds. This metabolism continued even throughout germination of the seed without reconversion of the metabolites to GA(5). It is concluded that "bound" GA(5) plays no part in the germination of dwarf pea seeds.
放射性赤霉素A(5)（(3)H-GA(5)）由赤霉酸合成。将其施用于在黑暗中生长的矮化豌豆时，平均有3%在48小时内转化为另一种酸性赤霉素。代谢产物的生物活性不能解释对施用的GA(5)的反应。因此，假定GA(5)本身具有生物活性。(3)H-GA(5)似乎未与豌豆嫩枝中的大分子形成稳定复合物。当注入矮化豌豆荚中时，(3)H-GA(5)很容易被成熟种子代谢为更易溶于水的物质和另外两种酸性化合物。这种代谢甚至在种子整个萌发过程中持续进行，代谢产物不会再转化为GA(5)。得出的结论是，“结合态”GA(5)在矮化豌豆种子萌发过程中不起作用。
