Durley R C, Sassa T, Pharis R P
Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4 Canada.
Plant Physiol. 1979 Aug;64(2):214-9. doi: 10.1104/pp.64.2.214.
Tritium-labeled gibberellin A(20) ([(3)H]GA(20)) was applied via the pedicel to immature pods and seeds of dwarf peas and three harvests were made at days 5, 10, and 23 (mature) after application. Of the five metabolites of [(3)H]GA(20), the three in highest yield were GA(29), an alpha,beta-unsaturated ketone, and a compound (B), whose structure was only tentatively assigned. The metabolic sequence GA(20) --> GA(29) --> compound B --> the ketone was indicated. The amount of [(3)H]GA(29) in both seeds and pods was highest at day 5 and declined to its lowest level at maturity. The amount of the [(3)H]ketone in the seed increased with time to its highest level at maturity. It is suggested that compound B and the ketone represent the major pathway of catabolism of GA(29), a 2beta-hydroxylated GA of low biological activity, and that the ketone is not metabolized, or only slowly metabolized, during seed maturation.
将氚标记的赤霉素A(20)（[(3)H]GA(20)）通过花梗施用于矮生豌豆未成熟的豆荚和种子上，并在施用后的第5天、第10天和第23天（成熟时）进行了三次收获。在[(3)H]GA(20)的五种代谢产物中，产量最高的三种分别是GA(29)、一种α,β-不饱和酮和一种化合物（B），其结构只是初步确定。显示出代谢顺序为GA(20)→GA(29)→化合物B→酮。种子和豆荚中[(3)H]GA(29)的含量在第5天最高，在成熟时降至最低水平。种子中[(3)H]酮的含量随时间增加，在成熟时达到最高水平。有人认为化合物B和酮代表了GA(29)（一种生物活性较低的2β-羟基化赤霉素）分解代谢的主要途径，并且在种子成熟过程中酮不会被代谢，或者仅被缓慢代谢。
