Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822.
Plant Physiol. 1970 Feb;45(2):148-52. doi: 10.1104/pp.45.2.148.
Density labeling with deuterium oxide, gel electrophoresis, and isopycnic equilibrium sedimentation were used to study the appearance and disappearance of individual peroxidases in the embryos of germinating barley. No detectable label was incorporated into those peroxidases which are present in the embryo of the dry seed and disappear during germination. Deuterium was incorporated into the additional peroxidases which appeared in the embryo during germination. This incorporation is not due to deuterium-hydrogen exchange into preformed proteins. The results indicate that the newly appearing peroxidases arise by synthesis during germination.
氘水标记、凝胶电泳和等密度平衡沉降法被用于研究萌发大麦胚中个别过氧化物酶的出现和消失。那些在干种子胚中存在并在萌发过程中消失的过氧化物酶没有被检测到标记。氘被掺入在萌发过程中出现在胚中的额外过氧化物酶中。这种掺入不是由于氘-氢交换到预先形成的蛋白质中。结果表明，新出现的过氧化物酶是在萌发过程中通过合成产生的。
