从天然来源中气相色谱法分离细胞分裂素：来自根癌土壤杆菌的6-(3-甲基-2-丁烯基氨基)嘌呤。
Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources: 6-(3-Methyl-2-butenylamino) purine from Agrobacterium Tumefaciens.
作者信息
Upper C D, Helgeson J P, Kemp J D, Schmidt C J
机构信息
Pioneering Research Laboratory, Crops Research Division, Agricultural Research Service, United States Department of Agriculture, Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706.
出版信息
Plant Physiol. 1970 May;45(5):543-7. doi: 10.1104/pp.45.5.543.
Abstract
Gas-liquid chromatographic retention times for 20 purines or purine nucleosides, 14 of which are highly active cytokinins, are reported. With one exception, all of the naturally occurring cytokinins are separated.Ethyl acetate extraction of yeast transfer RNA hydrolysates and of culture filtrates of Agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins for immediate gas-liquid chromatography. This procedure permitted the detection of 6-(3-methyl-2-butenylamino)-9-beta, d-ribofuranosylpurine in yeast transfer RNA extracts. An active cytokinin was isolated from A. tumefaciens culture filtrates and was tentatively identified as 6-(3-methyl-2-butenylamino)purine.
摘要
报道了20种嘌呤或嘌呤核苷的气液色谱保留时间，其中14种是高活性细胞分裂素。除了一个例外，所有天然存在的细胞分裂素都被分离出来。用乙酸乙酯萃取酵母转移RNA水解物和根癌土壤杆菌的培养滤液，可使天然存在的细胞分裂素充分浓缩，以便立即进行气液色谱分析。该方法可检测酵母转移RNA提取物中的6-(3-甲基-2-丁烯基氨基)-9-β，d-呋喃核糖基嘌呤。从根癌土壤杆菌培养滤液中分离出一种活性细胞分裂素，初步鉴定为6-(3-甲基-2-丁烯基氨基)嘌呤。
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