Vreman H J, Skoog F
Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1972 May;49(5):848-51. doi: 10.1104/pp.49.5.848.
Five cytokinin-active ribonucleosides have been isolated from the transfer RNA of 7-day-old green pea shoots (Pisum sativum L. var. Alaska). Ultraviolet spectroscopy and mass spectrometry have been used to identify 6-(3-methyl-2-butenylamino)-9-beta-d-ribofuranosylpurine, 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-beta- d-ribofuranosylpurine, and 6-(4-hydroxy-3-methyl-2-butenylamino)-9-beta-d-ribofuranosylpurine. The latter was separated into the cis- and trans-isomers by thin layer chromatography. The fifth cytokinin is indicated to be 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-d -ribofuranosylpurine on the basis of its chromatographic properties.
已从7日龄的绿豌豆嫩梢（豌豆阿拉斯加变种）的转移核糖核酸中分离出5种具有细胞分裂素活性的核糖核苷。利用紫外光谱法和质谱法鉴定出6-(3-甲基-2-丁烯基氨基)-9-β-D-呋喃核糖基嘌呤、6-(4-羟基-3-甲基-2-丁烯基氨基)-2-甲硫基-9-β-D-呋喃核糖基嘌呤和6-(4-羟基-3-甲基-2-丁烯基氨基)-9-β-D-呋喃核糖基嘌呤。后者通过薄层色谱法分离为顺式和反式异构体。根据其色谱性质，第五种细胞分裂素被确定为6-(3-甲基-2-丁烯基氨基)-2-甲硫基-9-β-D-呋喃核糖基嘌呤。
