Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan, Canada.
Plant Physiol. 1970 May;45(5):598-600. doi: 10.1104/pp.45.5.598.
Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used. The cells also grew well with l-glutamine as nitrogen source. The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium, but ammonium enhanced the activity of glutamate dehydrogenase. Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium.
在添加克雷布斯循环酸的情况下，大豆细胞悬浮培养物可以在以铵为唯一氮源的限定培养基上生长。与含有硝酸盐和铵盐的常规培养基相比，当使用柠檬酸盐、苹果酸盐、富马酸盐或琥珀酸盐的铵盐时，可获得令人满意的生长。当使用延胡索酸盐、酒石酸盐、醋酸盐、碳酸盐或硫酸盐的铵盐时，生长很少或没有发生。细胞也可以很好地利用 l-谷氨酰胺作为氮源生长。与在硝酸盐培养基上生长的细胞相比，谷氨酰胺合成酶和异柠檬酸脱氢酶（烟酰胺腺嘌呤二核苷酸磷酸）的比活较低，但铵盐增强了谷氨酸脱氢酶的活性。大豆、小麦和亚麻的细胞已经在铵柠檬酸盐培养基上培养了很长一段时间。
