光照对星状丝孢酵母呼吸作用的抑制
Inhibition of Respiration in Prototheca zopfii by Light.
作者信息
Epel B L, Butler W L
机构信息
Department of Biology, University of California at San Diego, La Jolla, California 92037.
出版信息
Plant Physiol. 1970 Jun;45(6):728-34. doi: 10.1104/pp.45.6.728.
Irradiation of cells of Prototheca zopfii with blue light inhibited the respiratory capacity of the cells. The inhibition of respiration was correlated with a photodestruction of cytochrome c(551), cytochrome b(559), and cytochrome a(3). Cytochrome c(549), cytochrome b(555), and cytochrome b(564) were unaffected by the irradiation treatment. The alpha-band of reduced cytochrome a was shifted from 599 to 603 nm by irradiation, an effect similar to that observed when methanol was added to nonirradiated cells. The presence of oxygen was required during irradiation for both photoinhibition of respiration and photodestruction of the cytochromes. Cytochrome a(3) was protected against photodestruction by cyanide. Photodestruction of these same cytochromes also occurred when washed mitochondria of P. zopfii were irradiated.
用蓝光照射魏氏原藻细胞会抑制细胞的呼吸能力。呼吸作用的抑制与细胞色素c(551)、细胞色素b(559)和细胞色素a(3)的光破坏有关。细胞色素c(549)、细胞色素b(555)和细胞色素b(564)不受照射处理的影响。照射使还原型细胞色素a的α带从599纳米移至603纳米，这一效应与向未照射细胞中添加甲醇时观察到的效应相似。照射过程中需要有氧气存在才能同时发生呼吸作用的光抑制和细胞色素的光破坏。氰化物可保护细胞色素a(3)不被光破坏。当对洗涤后的魏氏原藻线粒体进行照射时，同样这些细胞色素也会发生光破坏。
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