D'Aoust J Y, Giroux J, Baraan L R, Schneider H, Martin W G
J Bacteriol. 1974 Nov;120(2):799-804. doi: 10.1128/jb.120.2.799-804.1974.
Light above 400 nm had selective effects on Escherichia coli ML-308: several processes or enzymes were strongly inhibited, whereas others were relatively unaffected. There was a correlation between the inhibition of respiration and the inhibition of active uptake of glycine. However, phenylalanine uptake did not show such a correlation. The decrease in adenosine 5'-triphosphate level during the first few minutes of illumination resembled the inactivation kinetics of phenylalanine uptake. The results suggest that phenylalanine uptake may not depend greatly on oxidative energy and may depend on the adenosine 5'-triphosphate level. The results for glycine suggest either that its active uptake and respiration involve a common photosensitive component or alternately, that only the respiratory chain contains the photosensitive component, and that glycine uptake is coupled almost exclusively to respiration. The critical photochemical lesion does not involve d-lactate dehydrogenase, succinate dehydrogenase, or l-alpha-glycerophosphate dehydrogenase since their inactivation rate is markedly lower than that for respiration.
波长高于400纳米的光对大肠杆菌ML - 308有选择性作用：几个过程或酶受到强烈抑制，而其他一些则相对未受影响。呼吸抑制与甘氨酸主动摄取抑制之间存在相关性。然而，苯丙氨酸摄取并未表现出这种相关性。光照最初几分钟内三磷酸腺苷水平的下降类似于苯丙氨酸摄取的失活动力学。结果表明，苯丙氨酸摄取可能在很大程度上不依赖于氧化能，而可能取决于三磷酸腺苷水平。甘氨酸的结果表明，其主动摄取和呼吸要么涉及一个共同的光敏成分，要么另外，只有呼吸链含有光敏成分，并且甘氨酸摄取几乎完全与呼吸偶联。关键的光化学损伤不涉及d - 乳酸脱氢酶、琥珀酸脱氢酶或l - α - 甘油磷酸脱氢酶，因为它们的失活速率明显低于呼吸的失活速率。
