Department of Botany and Microbiology, Montana State University, Bozeman, Montana 59715.
Plant Physiol. 1970 Jul;46(1):126-35. doi: 10.1104/pp.46.1.126.
The variation in starch content in healthy and Puccinia striiformsi-infected wheat leaves was measured from 5 to 15 days after inoculation. The starch content of diseased leaves relative to healthy leaves decreased from 5 to 9 days, increased from 9 to 12 days to twice that of healthy leaves, and decreased from 12 to 15 days after inoculation. Electron micrographs of plant tissues indicated that the starch accumulated in the chloroplasts of host cells adjacent to fungal hyphae. Variations in sugar phosphates, ATP, and inorganic phosphate were measured during the infection process. ADP-glucose pyrophosphorylase was extracted and partially purified from healthy and diseased leaves. When proportionate concentrations of sugar phosphates and inorganic phosphate found in healthy and diseased leaves during the infection process were placed in the assay mixture, ADP-glucose pyrophosphorylase activity was similar to the pattern of starch accumulation and was almost the inverse of the variation observed in inorganic phosphate in diseased leaves during the infection process. A mechanism to explain the accumulation of starch is presented and discussed. This mechanism is based on the regulation of ADP-glucose pyrophosphorylase by changes in effector molecule concentrations during the infection process. Reasons for these changes are presented.
在接种后 5 至 15 天内，测量了健康和条锈菌感染的小麦叶片中淀粉含量的变化。与健康叶片相比，患病叶片的淀粉含量从第 5 天到第 9 天减少，从第 9 天到第 12 天增加到健康叶片的两倍，从第 12 天到第 15 天减少。植物组织的电子显微镜照片表明，淀粉在真菌菌丝相邻的宿主细胞的叶绿体中积累。在感染过程中测量了糖磷酸、ATP 和无机磷酸盐的变化。从健康和患病叶片中提取并部分纯化了 ADP-葡萄糖焦磷酸化酶。当将感染过程中健康和患病叶片中发现的糖磷酸和无机磷酸盐的比例浓度放入测定混合物中时，ADP-葡萄糖焦磷酸化酶活性与淀粉积累模式相似，与感染过程中患病叶片中无机磷酸盐的变化几乎相反。提出并讨论了一种解释淀粉积累的机制。该机制基于在感染过程中效应分子浓度变化对 ADP-葡萄糖焦磷酸化酶的调节。提出了这些变化的原因。
