菜豆叶片外植体脱落过程中纤维素酶的合成。
Synthesis of Cellulase during Abscission of Phaseolus vulgaris Leaf Explants.
机构信息
Department of Horticultural Science, University of California, Riverside, California 92502.
出版信息
Plant Physiol. 1970 Aug;46(2):194-9. doi: 10.1104/pp.46.2.194.
Abstract
When abscission in leaf explants from Phaseolus vulgaris, cultivar Red Kidney, was allowed to proceed while the explants were in (2)H(2)O, a 1.25% increase in the buoyant density of cellulase in a cesium chloride gradient was observed. These data indicate that the increase in cellulase activity during abscission is a result of the synthesis of new protein. Two differentially soluble forms of cellulase are present in the abscission zone. The form which is soluble only in a high salt buffer seems more closely related to the abscission process than the form which is soluble in dilute buffer. The correlation between changes in pull force and increase in cellulase activity and the effects of several hormones on cellulase activity are discussed.
摘要
当菜豆品种“红肾”的叶片外植体在 (2)H(2)O 中进行离区时，在铯氯化物梯度中观察到纤维素酶的浮力密度增加了 1.25%。这些数据表明，离区过程中纤维素酶活性的增加是新蛋白质合成的结果。在离区中有两种不同溶解性的纤维素酶。仅在高盐缓冲液中可溶的形式似乎比在稀缓冲液中可溶的形式更与离区过程密切相关。探讨了拉力变化与纤维素酶活性增加之间的相关性，以及几种激素对纤维素酶活性的影响。
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本文引用的文献
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Abscission: role of cellulase.脱落：纤维素酶的作用。
Plant Physiol. 1969 Mar;44(3):447-52. doi: 10.1104/pp.44.3.447.
2
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Plant Physiol. 1968 May;43(5):735-42. doi: 10.1104/pp.43.5.735.
3
Evidence for de novo synthesis of isocitratase and malate synthesis in germinating peanut cotyledons.发芽花生子叶中异柠檬酸裂合酶和苹果酸合成的从头合成证据。
Plant Physiol. 1968 Apr;43(4):660-4. doi: 10.1104/pp.43.4.660.
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Plant Physiol. 1967 Aug;42(8):1114-22. doi: 10.1104/pp.42.8.1114.
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