糖苷酶与豆芽下胚轴生长的关系。
Relation of glycosidases to bean hypocotyl growth.
机构信息
Department of Botany and Plant Pathology, Iowa State University, Ames, Iowa 50010.
出版信息
Plant Physiol. 1970 Sep;46(3):458-62. doi: 10.1104/pp.46.3.458.
Abstract
The enzymes beta-glucosidase, alpha-glucosidase, beta-galactosidase, alpha-galactosidase, and beta-xylosidase were detected in Phaseolus vulgaris L. var. Red Kidney bean hypocotyl tissue throughout the first 13 days of development with p-nitrophenyl glycosides as substrates. Activities of all enzymes except beta-glucosidase declined as a function of increasing tissue age. In contrast, beta-glucosidase activity increased rapidly 3 days after imbibition to a maximal activity at 5 days and then subsided to one-third the maximum by day 7. This activity peak immediately preceded the logarithmic phase of hypocotyl growth. This enzyme is strongly associated with cell walls during extraction, suggesting that it is wall-bound in situ. Various polysaccharide substrates were used to evaluate the specificity of this enzyme.
摘要
在豌豆下胚轴组织发育的最初 13 天中，用对硝基苯糖苷作为底物，检测到了β-葡萄糖苷酶、α-葡萄糖苷酶、β-半乳糖苷酶、α-半乳糖苷酶和β-木糖苷酶。除β-葡萄糖苷酶外，所有酶的活性都随着组织年龄的增加而下降。相比之下，β-葡萄糖苷酶的活性在吸水后 3 天迅速增加，在 5 天达到最大值，然后在第 7 天降至最大值的三分之一。这个活性高峰与下胚轴生长的对数期正好吻合。该酶在提取过程中与细胞壁强烈结合，表明它在原位与细胞壁结合。使用各种多糖底物来评估该酶的特异性。
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