Duncan G E, Little K Y, Koplas P A, Kirkman J A, Breese G R, Stumpf W E
Brain and Development Research Center, University of North Carolina, Chapel Hill 27599.
Brain Res. 1991 Oct 4;561(1):84-92. doi: 10.1016/0006-8993(91)90752-h.
The topography of beta-adrenergic receptors in the rat and human hippocampal formation was assessed by in vitro binding of 125I-pindolol to tissue sections. Marked differences were found in the distribution of beta-adrenergic receptors and in the relative amounts of beta 1 and beta 2 receptor subtypes in the two species. In the human, the highest receptor densities were present in the pyramidal cell layer and in the stratum lacunosum-moleculare. In the rat hippocampus, those regions contained the lowest densities of 125I-pindolol binding sites. The highest densities of beta-adrenergic receptors in the rat hippocampal formation were found in the ventral subiculum and in the entorhinal cortex. In contrast, in the human hippocampus, the subiculum and entorhinal cortex contained relatively low densities of the receptors. Competition studies with beta 1- and beta 2-selective antagonists revealed that beta 2-adrenergic receptors predominate in the human hippocampus and beta 1-adrenergic receptors predominate in the rat hippocampus. The marked species differences observed suggest that the pharmacological responsivity of the hippocampus to adrenergic agents and the role of noradrenaline in regulation of hippocampal function could be very different in rats compared to humans.
通过将125I-吲哚洛尔与组织切片进行体外结合，评估了大鼠和人类海马结构中β-肾上腺素能受体的分布情况。在这两个物种中，β-肾上腺素能受体的分布以及β1和β2受体亚型的相对含量存在显著差异。在人类中，最高的受体密度出现在锥体细胞层和分子层。在大鼠海马体中，这些区域的125I-吲哚洛尔结合位点密度最低。大鼠海马结构中β-肾上腺素能受体的最高密度出现在腹侧下托和内嗅皮质。相比之下，在人类海马体中，下托和内嗅皮质的受体密度相对较低。用β1和β2选择性拮抗剂进行的竞争研究表明，β2-肾上腺素能受体在人类海马体中占主导地位，而β1-肾上腺素能受体在大鼠海马体中占主导地位。观察到的显著物种差异表明，与人类相比，大鼠海马体对肾上腺素能药物的药理反应性以及去甲肾上腺素在调节海马体功能中的作用可能非常不同。
