Rainbow T C, Parsons B, Wolfe B B
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1585-9. doi: 10.1073/pnas.81.5.1585.
We have used quantitative autoradiography to localize in rat brain beta 1- and beta 2-adrenergic receptors. These receptors were labeled in vitro with 125I-labeled pindolol, an antagonist of beta-adrenergic receptors that binds nonselectively to both beta 1 and beta 2 subtypes. The selective inhibition of 125I-labeled pindolol binding with specific antagonists of beta 1 and beta 2 receptors allowed the visualization of beta-adrenergic receptor subtypes. High levels of beta 1 receptors were observed in the cingulate cortex, layers I and II of the cerebral cortex, the hippocampus, the Islands of Calleja, and the gelatinosus, mediodorsal, and ventral nuclei of the thalamus. High levels of beta 2 receptors were found in the molecular layer of the cerebellum, over pia mater, and in the central, paraventricular, and caudal lateral posterior thalamic nuclei. Approximately equal levels of beta 1 and beta 2 receptors occurred in the substantia nigra, the olfactory tubercle, layer IV of the cerebral cortex, the medial preoptic nucleus, and all nuclei of the medulla. The pronounced differences in the ratio of beta 1 to beta 2 receptors among brain regions suggests that the subtypes of beta-adrenergic receptors may play different roles in neuronal function.
我们运用定量放射自显影技术在大鼠脑中定位β1 - 和β2 - 肾上腺素能受体。这些受体在体外用125I标记的吲哚洛尔进行标记，吲哚洛尔是一种β - 肾上腺素能受体拮抗剂，它能非选择性地与β1和β2亚型结合。通过用β1和β2受体的特异性拮抗剂对125I标记的吲哚洛尔结合进行选择性抑制，得以观察到β - 肾上腺素能受体亚型。在扣带回皮质、大脑皮质的I层和II层、海马体、卡耶哈岛以及丘脑的胶状核、背内侧核和腹侧核中观察到高水平的β1受体。在小脑的分子层、软脑膜上方以及丘脑的中央核、室旁核和尾侧后外侧核中发现高水平的β2受体。在黑质、嗅结节、大脑皮质的IV层、内侧视前核以及延髓的所有核团中，β1和β2受体的水平大致相等。脑区中β1与β2受体比例的显著差异表明，β - 肾上腺素能受体亚型可能在神经元功能中发挥不同作用。
