用于微量测定血清中血管紧张素转换酶水平的双胺荧光底物。

Bimane fluorogenic substrates for microdetermination of angiotensin converting enzyme level in serum.

作者信息

Sato E, Hattori H, Nishikawa S, Kanaoka Y

机构信息

Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1991 Aug;39(8):2146-8. doi: 10.1248/cpb.39.2146.

DOI:10.1248/cpb.39.2146

PMID:1665758

Abstract

The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of nitrated aromatic amino acid. Bimane peptides containing nitrated amino acid (1a, b) were shown to be useful fluorogenic substrates for the assay of angiotensin I converting enzyme (ACE) from rabbit lung, similar to bimane substrate containing tryptophan (3) previously reported. Among these bimane substrates, substrate 3 was shown to be a potent fluorogenic substrate for microdetermination of the ACE level in human serum.

摘要

研究发现，在存在硝化芳香族氨基酸的情况下，9,10 - 二氧杂 - 顺 - 3,4,6,7 - 四甲基联二萘（联二萘）的荧光会被淬灭。含硝化氨基酸的联二萘肽（1a、b）被证明是用于检测兔肺血管紧张素I转化酶（ACE）的有效荧光底物，类似于先前报道的含色氨酸的联二萘底物（3）。在这些联二萘底物中，底物3被证明是用于微量测定人血清中ACE水平的有效荧光底物。