烟草细胞培养中 d-色氨酸向 l-色氨酸的转化。
The Conversion of d-Tryptophan to l-Tryptophan in Cell Cultures of Tobacco.
机构信息
Department of Biology, University of California, San Diego, La Jolla, California 92037.
出版信息
Plant Physiol. 1971 Apr;47(4):483-7. doi: 10.1104/pp.47.4.483.
Abstract
d-Tryptophan was converted to l-tryptophan in tissue cultures of tobacco, in whole cells treated with dimethylsulfoxide, and in cell-free extracts treated by Sephadex G-25 filtration. Evidence was obtained that tryptophanase, tryptophan pyrrolase, and transaminase activities were not involved. The data were best explained by the presence of a tryptophan racemase as the enzyme catalyzing the reaction. The possible role of d-tryptophan in the biosynthesis of indoleacetic acid is discussed.
摘要
d-色氨酸在烟草组织培养物、用二甲基亚砜处理的完整细胞以及用 Sephadex G-25 过滤处理的无细胞提取物中被转化为 l-色氨酸。有证据表明，色氨酸酶、色氨酸吡咯酶和转氨酶活性不参与该反应。这些数据最好通过存在一种色氨酸外消旋酶作为催化该反应的酶来解释。还讨论了 d-色氨酸在吲哚乙酸生物合成中的可能作用。
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