Department of Botany, University of California, Berkeley, California 94720.
Plant Physiol. 1972 Jan;49(1):97-101. doi: 10.1104/pp.49.1.97.
Chloroplast replication was induced in mature tobacco leaf tissue (Nicotiana tabacum L.) by culturing leaf discs on a sterile medium composed of salts and sucrose. Chloroplast replicaton is greatly enhanced by the addition of kinetin to this medium. Kinetin also enhances cell enlargement, but cell division does not occur. Chloroplast replication is nonsynchronous and proceeds most rapidly when the cell enlargement rate decreases. Chloroplast replication is light-dependent, but cell enlargement occurs in both light and dark. Chloroplast replication resumes when discs cultured in the dark are returned to the light. It appears that chloroplast replication is related to cell expansion. The possibility of inducing synchronous replication of chloroplasts in tobacco cells is discussed.
通过在含有盐和蔗糖的无菌培养基上培养叶圆盘，诱导成熟烟草叶片组织（Nicotiana tabacum L.）中的叶绿体复制。向培养基中添加激动素可大大增强叶绿体的复制。激动素还可增强细胞增大，但不会发生细胞分裂。叶绿体复制不同步，当细胞增大率降低时，复制速度最快。叶绿体复制依赖于光，但细胞增大可在光照和黑暗中发生。当在黑暗中培养的圆盘返回光照时，叶绿体复制会恢复。看来叶绿体复制与细胞扩张有关。讨论了诱导烟草细胞中叶绿体同步复制的可能性。
