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小蘗嘧啶代谢:II. 高等植物胞苷脱氧核苷对质体复制的专一性抑制。

Pyrimidine Metabolism in Lemna minor: II. Specific Inhibition of Plastid Replication in a Higher Plant Cytidine Deoxyriboside.

机构信息

Plant Science Department, University of Delaware, Newark, Delaware 19711.

出版信息

Plant Physiol. 1979 Jun;63(6):979-83. doi: 10.1104/pp.63.6.979.

DOI:10.1104/pp.63.6.979
PMID:16660889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC542956/
Abstract

Short term photoheterotrophic growth of Lemna minor in the presence of 100 micrograms per milliliter (440 micromolar) cytidine deoxyriboside bleaches the fronds in the absence of effect upon growth rate (Frick 1978 Plant Physiol 61: 989-992). Serial sections of mesophyll cells of green and bleached fronds were compared in the light microscope. The number of plastids in cells of comparable size was reduced during bleaching by more than 50%. The total and average plastid profile area per section in the series was reduced on the order of 70%, and the summed plastid profile area over the entire cell was reduced about 90%. Thus, cytidine deoxyri-boside-bleached mesophyll cells contained fewer identifiable plastids (50%) of smaller volume (10-30%) than the green control cells.

摘要

短期异养生长的浮萍在存在 100 微克/毫升(440 微摩尔)的胞苷脱氧核苷漂白的羽片在不影响生长速度(弗里克 1978 年植物生理学 61:989-992)。在光镜下比较了绿色和漂白羽片的叶肉细胞的连续切片。在漂白过程中,可比大小的细胞中的质体数量减少了 50%以上。整个系列中每个部分的总和平均质体轮廓面积减少了约 70%,整个细胞的总和质体轮廓面积减少了约 90%。因此,胞苷脱氧核糖苷漂白的叶肉细胞比绿色对照细胞含有更少可识别的质体(50%)和更小的体积(10-30%)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b2c/542956/20bf94655923/plntphys00127-0003-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b2c/542956/20bf94655923/plntphys00127-0003-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b2c/542956/20bf94655923/plntphys00127-0003-a.jpg

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本文引用的文献

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Analysis of complex vascular systems in plants: optical shuttle method.植物复杂维管系统分析:光学穿梭法
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Pyrimidine Metabolism in Lemna minor: I. Functional Compartmentation of Chloroplast Pyrimidine Metabolism in a Higher Plant.小蘗叶中嘧啶代谢:I. 高等植物叶绿体嘧啶代谢的功能区室化。
Plant Physiol. 1978 Jun;61(6):989-92. doi: 10.1104/pp.61.6.989.
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A low-viscosity epoxy resin embedding medium for electron microscopy.一种用于电子显微镜的低粘度环氧树脂包埋介质。
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Synchronous division of plastids during the greening of cut leaves of maize.
Nat New Biol. 1973 Apr 4;242(118):158-60. doi: 10.1038/newbio242158a0.
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Distribution of DNA in dividing spinach chloroplasts.菠菜叶绿体分裂过程中DNA的分布
Nature. 1974 Oct 18;251(5476):641-2. doi: 10.1038/251641a0.
10
Macromolecular physiology of plastids. IX. Development of plastid membranes during greening of dark-grown barley seedlings.质体的大分子生理学。IX. 黑暗生长的大麦幼苗绿化过程中质体膜的发育
J Cell Sci. 1974 Jun;15(1):31-55. doi: 10.1242/jcs.15.1.31.