Raschke K
Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.
Plant Physiol. 1972 Feb;49(2):229-34. doi: 10.1104/pp.49.2.229.
Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was 3 seconds. The velocity of stomatal closing increased with CO(2) concentration and approached its maximal value between 10(3) and 10(4) microliters CO(2) per liter of air. The CO(2) concentration at which the closing velocity reached half its maximal value was approximately 200 microliters CO(2) per liter of air, both in the light and in darkness. This indicates that the mechanism of stomatal responses to CO(2) is the same in both light regimes and that the range of stomatal sensitivity to changes in CO(2) concentration coincides with the range of CO(2) concentrations known to occur in the intercellular spaces of illuminated leaves.
使用气流气孔计记录了玉米叶片切片中气孔对从不含二氧化碳的空气转变为含二氧化碳的空气的关闭运动。对二氧化碳的反应很快；每升空气中施加300微升二氧化碳与气孔反应开始之间的最短延迟为3秒。气孔关闭速度随二氧化碳浓度增加而增加，并在每升空气中10³至10⁴微升二氧化碳之间接近其最大值。无论在光照还是黑暗条件下，关闭速度达到其最大值一半时的二氧化碳浓度约为每升空气中200微升二氧化碳。这表明在两种光照条件下，气孔对二氧化碳的反应机制相同，并且气孔对二氧化碳浓度变化的敏感范围与已知在光照叶片细胞间隙中出现的二氧化碳浓度范围一致。
