Rockefeller University, New York, New York 10021.
Plant Physiol. 1972 Mar;49(3):388-92. doi: 10.1104/pp.49.3.388.
A sensitive luminometer is used to measure directly the low rates of oxygen evolution during greening of etiolated barley (Hordeum vulgare L. var. Wong) leaves. Oxygen evolution is measured in leaf segments infiltrated with p-benzoquinone. When illuminated, these leaves do not produce significant amounts of oxygen until the end of the lag phase of chlorophyll synthesis. Chlorophyll is increased by feeding delta-aminolevulinic acid to leaves in the lag phase, but this does not cause an earlier appearance of photosynthesis. Chloramphenicol, and to a lesser extent cycloheximide, when fed to leaves together with delta-aminolevulinic acid, strongly inhibit the development of oxygen evolution in the light while only slightly inhibiting chlorophyll synthesis. The ability to evolve oxygen develops to only a slight extent in darkness, even in the presence of high levels of chlorophyll.We conclude that the development of photosystem II is limited by the synthesis of proteins in both the cytoplasm and the plastid, not by chlorophyll synthesis. Prolonged illumination is necessary for the development of oxygen evolution.
一种灵敏的光度计被用于直接测量黄化大麦（Hordeum vulgare L. var. Wong）叶片在变绿过程中氧气释放的低速率。氧气释放是在被对苯醌浸润的叶片片段中测量的。当被照射时，这些叶片在叶绿素合成的滞后阶段结束之前不会产生大量的氧气。在滞后阶段向叶片中添加 δ-氨基乙酰丙酸可以增加叶绿素，但这不会导致光合作用更早出现。当与 δ-氨基乙酰丙酸一起喂食氯霉素时，以及在较小程度上喂食环己酰亚胺时，它们强烈抑制光下氧气释放的发展，而仅轻微抑制叶绿素合成。即使在高水平的叶绿素存在下，黑暗中氧气的释放能力也只能得到轻微的发展。我们得出结论，光合作用系统 II 的发展受到细胞质和质体中蛋白质合成的限制，而不是由叶绿素合成所限制。氧气释放的发展需要长时间的光照。
