Department of Biology, State University of New York, Binghamton, New York 13901.
Plant Physiol. 1971 Apr;47(4):545-50. doi: 10.1104/pp.47.4.545.
Callus cultures were obtained from five selenium accumulator and three nonaccumulator species of Astragalus. Their morphological characteristics and their growth responses to light, sucrose, kinetin, and 2,4-dichlorophenoxyacetic acid are described. Calluses derived from accumulator species characteristically retained their tolerance to high concentrations of selenate and selenite, whereas calluses derived from nonaccumulator species were markedly inhibited by these two forms of selenium. Competition between sulfate and selenate was demonstrated. The two types of calluses could not be distinguished on the basis of (75)Se-labeled selenate or selenite uptake. Neutron activation analysis failed to show differences in selenium content between the two types of calluses grown on media to which no selenium had been added.
从五种富硒黄芪和三种非富硒黄芪中获得愈伤组织培养物。描述了它们的形态特征及其对光、蔗糖、激动素和 2,4-二氯苯氧乙酸的生长反应。从富硒种中获得的愈伤组织特征性地保持了对高浓度硒酸盐和亚硒酸盐的耐受性，而从非富硒种中获得的愈伤组织则明显受到这两种形式的硒的抑制。硫酸盐和硒酸盐之间存在竞争。两种类型的愈伤组织不能根据 (75)Se 标记的硒酸盐或亚硒酸盐摄取来区分。中子激活分析未能显示在未添加硒的培养基上生长的两种类型的愈伤组织之间的硒含量有差异。
