离体花粉管生长抑制测量。
In vitro measurement of pollen tube growth inhibition.
机构信息
Federal Experiment Station, Plant Science Research Division, Agricultural Research Service, United States Department of Agriculture, Mayaguez, Puerto Rico.
出版信息
Plant Physiol. 1972 Jun;49(6):924-5. doi: 10.1104/pp.49.6.924.
A method for estimating inhibition of pollen tube growth was developed. Pollen is placed in straight lines on an agar surface where it responds uniformly and predictably to aqueous solutions of germination-inhibiting substances located in wells at the ends of the lines. A scale of ratings, roughly corresponding to serial, doubled concentrations of inhibiting substances, was devised. Water-soluble organic solvents are relatively noninhibitory, salts are variable, and metabolic inhibitors have strong inhibitory effects. Pollens differ in their susceptibility to inhibition and in their response to particular substances.
一种评估花粉管生长抑制的方法已经建立。花粉被放置在琼脂表面的直线上，在那里它们对位于直线末端井中的发芽抑制物质的水溶液均匀且可预测地作出反应。设计了一个大致对应于抑制物质连续加倍浓度的评级量表。水溶性有机溶剂的抑制作用相对较弱，盐类的抑制作用变化较大，代谢抑制剂的抑制作用较强。不同的花粉对抑制作用的敏感性不同，对特定物质的反应也不同。
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