豌豆和小麦的离体黄化质体和叶绿体的蛋白质合成以及氯霉素和环己酰亚胺的作用。
Protein synthesis by isolated etioplasts and chloroplasts from pea and wheat and the effects of chloramphenicol and cycloheximide.
作者信息
Reger B J, Smillie R M, Fuller R C
机构信息
Biology Division, Oak Ridge National Laboratory, The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830.
出版信息
Plant Physiol. 1972 Jul;50(1):19-23. doi: 10.1104/pp.50.1.19.
Abstract
Etioplasts capable of incorporating (14)C-leucine into protein have been isolated from dark-grown pea and wheat plants. The requirements for leucine incorporation for etioplasts were similar to those for chloroplasts. An ATP-generating system, Mg(2+), and GTP were required. The amino-acid-incorporation activity of etioplasts from wheat was comparable to that of chloroplasts on an RNA basis, whereas the activity of pea etioplasts was about 50% of the activity of pea chloroplasts. The incorporation of leucine into protein by etioplasts and chloroplasts from pea and wheat was inhibited by chloramphenicol, and to a slight extent by cycloheximide.
摘要
已从黑暗中生长的豌豆和小麦植株中分离出能够将（14）C-亮氨酸掺入蛋白质的黄化质体。黄化质体掺入亮氨酸的条件与叶绿体相似。需要一个ATP生成系统、Mg(2+)和GTP。从小麦黄化质体的RNA基础来看，其氨基酸掺入活性与叶绿体相当，而豌豆黄化质体的活性约为豌豆叶绿体活性的50%。豌豆和小麦的黄化质体及叶绿体将亮氨酸掺入蛋白质的过程受到氯霉素的抑制，并在一定程度上受到环己酰亚胺的抑制。
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本文引用的文献
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