Plant Physiology Unit, Commonwealth Scientific and Industrial Research Organization, Division of Food Preservation, Ryde and School of Biological Sciences, The University of Sydney, N.S.W. 2006, Australia.
Plant Physiol. 1971 Mar;47(3):385-8. doi: 10.1104/pp.47.3.385.
Dark-grown pea seedlings (Pisum sativum L.) were irradiated for a short period each day with low intensity red light (662 nm), red light immediately followed by far red light (730 nm), or far red light alone. Other plants were transferred to a white light regime (14 hours light/10 hours dark). There was no change in the amount of RNA in the tissue on a fresh weight basis after the various treatments. However, compared with dark-grown seedlings, those plants irradiated with red light showed an increase in the net RNA content per stem apex. In addition there was a two- to three-fold increase in ribosomal RNA of the etioplasts relative to the total ribosomal RNA. These increases were comparable to those found in plants grown in the white light regime. The changes were much smaller if the dark-grown plants were irradiated either with red light followed by far red light, or with far red light alone. Thus continuous light is not essential for the production of ribosomal RNA in plastids, and the levels of ribosomal RNA found in chloroplasts can also be attained in etioplasts of pea leaves in the dark provided the leaf phytochrome is maintained in its active form.
暗培养的豌豆幼苗（Pisum sativum L.）每天用低强度的红光（662nm）短时间照射，红光后立即用远红光（730nm）照射，或单独用远红光照射。其他植物被转移到白光环境（14 小时光照/10 小时黑暗）。经过各种处理后，组织的 RNA 含量在鲜重基础上没有变化。然而，与暗培养的幼苗相比，那些用红光照射的幼苗，其茎尖的净 RNA 含量增加。此外，相对于总核糖体 RNA，质体中的核糖体 RNA 增加了两到三倍。这些增加与在白光环境中生长的植物相似。如果暗培养的植物用红光后紧接着远红光照射，或者只用远红光照射，这些变化就小得多。因此，连续光照不是质体中核糖体 RNA 产生的必要条件，在黑暗中，只要叶类黄酮保持其活性形式，在叶绿体中发现的核糖体 RNA 水平也可以在豌豆叶片的黄化质体中达到。
