从不同成熟度的烟草叶片中用洗涤剂提取酶。
Detergent extraction of enzymes from tobacco leaves varying in maturity.
作者信息
De Jong D W
机构信息
Plant Science Research Division, Agricultural Research Service, United States Department of Agriculture, Oxford, North Carolina 27565.
出版信息
Plant Physiol. 1972 Dec;50(6):733-7. doi: 10.1104/pp.50.6.733.
Abstract
Enzyme activities of tobacco leaves were compared in detergent extracts. Highest levels of chlorogenic acid oxidase, malate-NAD oxidoreductase, and acid phosphatase were obtained from bud tissues. Peroxidase activity was least in young leaves and highest in senescent leaves yellowed with Ethrel. Peroxidase zymograms obtained by means of disc electrophoresis showed differences in isozyme composition among all five samples examined. Although protease was found in material extracted from buds, upper, middle, and lower leaf positions, none could be demonstrated in Ethrel-treated mature leaves.
摘要
在去污剂提取物中比较了烟草叶片的酶活性。从芽组织中获得了最高水平的绿原酸氧化酶、苹果酸-NAD氧化还原酶和酸性磷酸酶。过氧化物酶活性在幼叶中最低，在经乙烯利处理变黄的衰老叶片中最高。通过圆盘电泳获得的过氧化物酶同工酶图谱显示，在所检测的所有五个样品中，同工酶组成存在差异。尽管在从芽、上部、中部和下部叶位提取的材料中发现了蛋白酶，但在经乙烯利处理的成熟叶片中未检测到蛋白酶。
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