Department of Botany and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma 74074.
Plant Physiol. 1973 Jan;51(1):128-35. doi: 10.1104/pp.51.1.128.
Several factors which influence the translocation patterns of stem-injected indoleacetic acid, 2,4-dichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxyacetic acid in bean seedlings (Phaseolus vulgaris L. cv. Stringless Greenpod) were characterized. The acropetal translocation of auxin from the site of injection is markedly sensitive to concentration in the range of 1.0 to 5.0 micrograms per plant. The antiauxin p-chlorophenoxyisobutyric acid enhanced translocation of 2,4,5-trichlorophenoxyacetic acid to the growing shoots and primary leaves. Translocation to the roots was unaffected by p-chlorophenoxyisobutyric acid while leaching of 2,4,5-trichlorophenoxyacetic acid into the nutrient solution was enhanced slightly. Steam girdling experiments revealed that translocation to the primary leaves was in the xylem. The protein synthesis inhibitor, cycloheximide, inhibited accumulation of 2,4,5-trichlorophenoxyacetic acid in young shoots, epicotyls, and roots and enhanced accumulation in the primary leaves. The relative exchangeability of auxin between xylem and phloem is discussed in terms of regulation of auxin movements in intact bean seedlings.
研究了影响注入吲哚乙酸、2,4-二氯苯氧乙酸和 2,4,5-三氯苯氧乙酸在菜豆（Phaseolus vulgaris L. cv. Stringless Greenpod）幼苗中转运模式的几个因素。从注射部位到顶端的生长素的向顶转运对 1.0 到 5.0 微克/株范围内的浓度明显敏感。抗生长素对氯苯氧异丁酸增强了 2,4,5-三氯苯氧乙酸向生长的嫩枝和初生叶的转运。对氯苯氧异丁酸对向根的转运没有影响，而 2,4,5-三氯苯氧乙酸进入营养液中的浸出略有增加。蒸汽环割实验表明，向初生叶的转运是在木质部中进行的。蛋白质合成抑制剂环己酰亚胺抑制了 2,4,5-三氯苯氧乙酸在嫩枝、上胚轴和根中的积累，并增强了在初生叶中的积累。根据完整菜豆幼苗中生长素运动的调节，讨论了生长素在木质部和韧皮部之间的相对可交换性。
