Gruffat D, Gonzalvez S, Mauchamp J, Chabaud O
INSERM U 270, Faculté de Médecine Nord, Marseilles, France.
Mol Cell Endocrinol. 1991 Oct;81(1-3):195-203. doi: 10.1016/0303-7207(91)90218-h.
Phenol red, commonly used as a pH indicator in tissue culture media, is known to possess estrogenic properties. We investigated the effect of phenol red on the process of thyroglobulin iodination which occurs only at the apical surface of porcine thyroid cells when cultured in porous bottom chambers. When phenol red was added simultaneously to both compartments (apical and basolateral), separated by the polarized monolayer, thyroglobulin iodination was inhibited by about 86% without any effect on thyroglobulin secretion and apical iodine concentration. When phenol red was added separately to either the apical or basal media, inhibition was 68% and 43%, respectively. A large amount of phenol red which was introduced into the basal medium crossed through the monolayer. Thus, inhibition was dependent upon the concentration of phenol red present in the apical compartment. A maximal inhibition was observed from 30 microM apical concentration. Phenol red acts as a substrate for thyroperoxidase in the iodination reaction.
酚红常用于组织培养基中的pH指示剂，已知具有雌激素特性。我们研究了酚红对甲状腺球蛋白碘化过程的影响，当在多孔底部培养室中培养时，该过程仅发生在猪甲状腺细胞的顶端表面。当酚红同时添加到由极化单层分隔的两个区室（顶端和基底外侧）时，甲状腺球蛋白碘化受到约86%的抑制，而对甲状腺球蛋白分泌和顶端碘浓度没有任何影响。当酚红分别添加到顶端或基底培养基中时，抑制率分别为68%和43%。引入基底培养基中的大量酚红穿过了单层。因此，抑制作用取决于顶端区室中酚红的浓度。在顶端浓度为30微摩尔时观察到最大抑制作用。酚红在碘化反应中作为甲状腺过氧化物酶的底物。
