Department of Chemistry, University of Colorado, Boulder, Colorado 80302.
Plant Physiol. 1973 Jan;51(1):188-97. doi: 10.1104/pp.51.1.188.
Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the fragments extracted from these walls by urea and alkali provide evidence for a covalent connection between the xyloglucan and pectic polysaccharides. Fragments released by a protease from endopolygalacturonase-endoglucanase-pretreated cell walls provide evidence for a covalent connection between the pectic polysaccharides and the structural protein of the cell wall. Based on these interconnections and the strong binding which occurs between the xyloglucan and cellulose, a tentative structure of the cell wall is proposed.
降解酶已被用于获得悬浮培养的枫细胞的分离细胞壁的定义片段。这些片段已被纯化并进行了结构表征。用纯化的内切葡聚糖酶从内切聚半乳糖醛酸酶预处理的细胞壁中释放的片段以及用尿素和碱从这些细胞壁中提取的片段为木葡聚糖和果胶多糖之间的共价连接提供了证据。用蛋白酶从内切聚半乳糖醛酸酶-内切葡聚糖酶预处理的细胞壁中释放的片段为果胶多糖和细胞壁结构蛋白之间的共价连接提供了证据。基于这些连接以及木葡聚糖与纤维素之间的强结合，提出了细胞壁的一个暂定结构。
