2-丁烯二酸二丁酯、2-苯基琥珀酸、丁烯二酸苄酯和对碘代苄烯二酸对绿豆线粒体氧化底物的影响。
Effects of 2-Butylmalonate, 2-Phenylsuccinate, Benzylmalonate, and p-Iodobenzylmalonate on the Oxidation of Substrates by Mung Bean Mitochondria.
机构信息
Department of Biochemistry, University of Toronto, Toronto 5, Ontario, Canada.
出版信息
Plant Physiol. 1973 Feb;51(2):225-8. doi: 10.1104/pp.51.2.225.
The effect of inhibitors of carboxylic acid anion transport on the oxidation of substrates by mung bean (Phaseolus aureus) mitochondria was investigated. The oxidation of malate in the presence of either glutamate or cysteine sulfinate was inhibited by 2-butylmalonate, 2-phenylsuccinate, benzylmalonate, and p-iodobenzylmalonate in both intact and broken mitochondria. The oxidation of succinate, on the other hand, was inhibited in intact but not in broken mitochondria. The oxidation of reduced nicotinamide adenine dinucleotide was inhibited only by p-iodobenzylmalonate. This inhibition occurred only in coupled mitochondria and could be reversed by the addition of adenosine diphosphate.These results suggest that the compounds studied inhibit the entry of succinate and probably also of malate into mung bean mitochondria. All four compounds inhibit the enzyme (s) which oxidize malate while p-iodobenzylmalonate also interferes with oxidative phosphorylation.
研究了羧酸阴离子转运抑制剂对绿豆（Phaseolus aureus）线粒体氧化底物的影响。在存在谷氨酸或半胱氨酸亚磺酸盐的情况下，2-丁烯二酸、2-苯基琥珀酸、苄基琥珀酸和对碘苄基琥珀酸均可抑制完整和破碎线粒体中苹果酸的氧化。另一方面，琥珀酸的氧化仅在完整线粒体中被抑制，而在破碎线粒体中则不受抑制。还原型烟酰胺腺嘌呤二核苷酸的氧化仅被对碘苄基琥珀酸抑制。这种抑制仅发生在偶联线粒体中，并可通过添加二磷酸腺苷逆转。这些结果表明，研究中使用的化合物抑制琥珀酸和可能还有苹果酸进入绿豆线粒体。所有四种化合物均抑制氧化苹果酸的酶（s），而对碘苄基琥珀酸还会干扰氧化磷酸化。
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