Gutteridge J M
Department of Anaesthesia and Intensive Care, Royal Brompton Hospital, London, United Kingdom.
Free Radic Biol Med. 1991;11(4):401-6. doi: 10.1016/0891-5849(91)90157-x.
When a ferric citrate complex is prepared from citric acid and ferric chloride, and the pH value left unchanged, a reduction of the iron moiety takes place. Within several hours a substantial yield of ferrous ions can be detected in the solution. When placed in a phosphate buffer pH 7.0 with a suitable detector molecule, oxidative damage to the detector molecule can be observed. Thus, deoxyribose is degraded with the release of thiobarbituric acid-reactive material and benzoate is hydroxylated to form fluorescent dihydroxy products. Damage can be prevented by scavengers of the hydroxyl radical such as mannitol, formate the thiourea, by catalase and by the protein caeruloplasmin, suggesting that Fenton chemistry occurs leading to the formation of hydroxyl radicals.
当由柠檬酸和氯化铁制备柠檬酸铁络合物且pH值保持不变时，铁部分会发生还原。在数小时内，溶液中可检测到大量亚铁离子。当置于含有合适检测分子的pH 7.0磷酸盐缓冲液中时，可观察到对检测分子的氧化损伤。因此，脱氧核糖降解并释放出硫代巴比妥酸反应性物质，苯甲酸酯被羟基化形成荧光二羟基产物。羟基自由基清除剂如甘露醇、甲酸盐、硫脲、过氧化氢酶和蛋白质铜蓝蛋白可防止损伤，这表明发生了芬顿化学反应导致羟基自由基的形成。
