Arts and Sciences Faculty, Department of Biology, Gazi University, 06500, Ankara, Turkey,
Cytotechnology. 2008 Feb;56(2):137-44. doi: 10.1007/s10616-008-9137-0. Epub 2008 Feb 21.
Clastogenic properties of the food additive citric acid, commonly used as an antioxidant, were analysed in human peripheral blood lymphocytes. Citric acid induced a significant increase of chromosomal aberrations (CAs) at all the concentrations and treatment periods tested. Citric acid significantly decreased mitotic index (MI) at 100 and 200 mug ml(-1) concentrations at 24 h, and in all concentrations at 48 h. However, it did not decrease the replication index (RI) significantly. Citric acid also significantly increased sister chromatid exchanges (SCEs) at 100 and 200 mug ml(-1) concentrations at 24 h, and in all concentrations at 48 h. This chemical significantly increased the micronuclei frequency (MN) compared to the negative control. It also decreased the cytokinesis-block proliferation index (CBPI), but this result was not statistically significant.
食品添加剂柠檬酸具有致突变性，通常用作抗氧化剂。本研究分析了柠檬酸对人外周血淋巴细胞的致突变性。在所有测试的浓度和处理时间下，柠檬酸均显著增加了染色体畸变（CAs）的数量。在 24 小时时，柠檬酸在 100 和 200μg/ml 浓度下显著降低了有丝分裂指数（MI），在所有浓度下均显著降低了 48 小时时的有丝分裂指数（MI）。然而，它并没有显著降低复制指数（RI）。在 24 小时时，柠檬酸在 100 和 200μg/ml 浓度下显著增加了姐妹染色单体交换（SCE），在所有浓度下均显著增加了 48 小时时的姐妹染色单体交换（SCE）。与阴性对照组相比，该化学物质显著增加了微核频率（MN）。它还降低了胞质分裂阻断增殖指数（CBPI），但这一结果没有统计学意义。
