在萌发的蓖麻种子的非光合胚乳中存在核酮糖 1,5-二磷酸羧化酶。
The presence of ribulose 1,5-diphosphate carboxylase in the nonphotosynthetic endosperm of germinating castor beans.
机构信息
Department of Plant Sciences, Texas A & M University, College Station, Texas 77843.
出版信息
Plant Physiol. 1973 Apr;51(4):755-9. doi: 10.1104/pp.51.4.755.
Abstract
Ribulose 1,5-diphosphate carboxylase was detected in extracts of germinating castor bean (Ricinus communis var. Hale) endosperms. This is the first report of this enzyme in a nonphotosynthetic (no chlorophyll) plant tissue. Radioactive 3-phosphoglyceric acid has been identified as the principle product resulting from the enzymatic condensation of (14)C-bicarbonate and ribulose-1,5-diP in endosperm extracts. The Km values of bicarbonate and ribulose-1,5-diP for the endosperm carboxylase are 1.14 x 10(-2)m and 7.5 x 10(-5)m, respectively. The carboxylase activity peaks at 4 days in endosperms of castor beans germinated in the dark. The specific activity of the carboxylase at this stage of germination is 4.3 mumoles of 3-phosphoglycerate formed/mg protein.hr. The presence of ribulose-1,5-diP carboxylase and other enzymes of the reductive pentose phosphate pathway show the potential of this pathway in castor bean endosperms.
摘要
在萌发的蓖麻（Ricinus communis var. Hale）胚乳提取物中检测到核酮糖 1,5-二磷酸羧化酶。这是该酶在非光合作用（无叶绿素）植物组织中的首次报道。放射性 3-磷酸甘油酸已被鉴定为（14）C-碳酸氢盐和核酮糖-1,5-二磷酸在胚乳提取物中酶缩合的主要产物。胚乳羧化酶对碳酸氢盐和核酮糖-1,5-二磷酸的 Km 值分别为 1.14 x 10(-2)m 和 7.5 x 10(-5)m。在黑暗中发芽的蓖麻胚乳中，羧化酶活性在第 4 天达到峰值。在这个发芽阶段，羧化酶的比活为 4.3 微摩尔 3-磷酸甘油酸形成/mg 蛋白。hr。核酮糖 1,5-二磷酸羧化酶和还原戊糖磷酸途径的其他酶的存在表明该途径在蓖麻胚乳中具有潜力。
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