Towill L R, Ikuma H
Department of Botany, University of Michigan, Ann Arbor, Michigan 48104.
Plant Physiol. 1973 May;51(5):973-8. doi: 10.1104/pp.51.5.973.
Light stimulates the germination of spores of the fern Onoclea sensibilis L. At high dosages, broad band red, far red, and blue light promote maximal germination. Maximal sensitivity to these spectral regions is attained from 6 to 48 hours of dark presoaking, and all induced rapid germination after a lag of 30 to 36 hours. Maximal germination is attained approximately 70 hours after irradiation. Dose response curves suggest log linearity. The action spectrum to cause 50% germination shows that spores are most sensitive to irradiation in the red region (620-680 nm) with an incident energy less than 1000 ergs cm(-2); sensitivity decreases towards both shorter and longer wavelengths. Although the action spectrum is suggestive of phytochrome involvement, photoreversibility of germination between red and far red light has not been demonstrated with Onoclea spores. An absorption spectrum of the intact spores reveals the presence of chlorophylls and carotenoids. Since the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea does not inhibit germination, it is concluded that photosynthesis does not play a role in the germination process.
光刺激敏感鳞毛蕨（Onoclea sensibilis L.）孢子的萌发。在高剂量下，宽带红光、远红光和蓝光可促进最大程度的萌发。在黑暗预浸泡6至48小时后，对这些光谱区域的敏感性达到最大，并且在30至36小时的延迟后，所有这些光都能诱导快速萌发。照射后约70小时达到最大萌发率。剂量反应曲线呈对数线性关系。导致50%萌发的作用光谱表明，孢子对红光区域（620 - 680纳米）的照射最为敏感，入射能量小于1000尔格/平方厘米；对较短和较长波长的敏感性均降低。尽管作用光谱表明可能涉及光敏色素，但尚未证明敏感鳞毛蕨孢子在红光和远红光之间的萌发具有光可逆性。完整孢子的吸收光谱显示存在叶绿素和类胡萝卜素。由于3 -（3,4 - 二氯苯基）-1,1 - 二甲基脲的存在并不抑制萌发，因此得出结论，光合作用在萌发过程中不起作用。
