Department of Biology, University of Tennessee at Chattanooga, Chattanooga, Tennessee 37401.
Plant Physiol. 1977 Apr;59(4):756-8. doi: 10.1104/pp.59.4.756.
Regulation of spore germination in the fern Onoclea sensibilis L. was investigated by applying CO(2) alone and in combination with ethylene. Sterile spores were sown aseptically on Knops solution in loosely capped culture tubes, enclosed individually in 2-liter chambers, and grown under continuous white light. When maintained in enclosed containers with the ethylene-absorbent mercuric perchlorate and with atmospheres enriched up to 2% CO(2) (v/v), spores germinated without any inhibition. Higher levels of applied CO(2) were progressively inhibitory. Inhibition by CO(2) was reversible. When CO(2) was permitted to escape and spores were exposed subsequently to ambient laboratory air, recovery from inhibition occurred within 48 hours. Also, inhibition by CO(2) was specific, since the same degree of inhibition resulted regardless of whether spores were treated with exogenous CO(2) for 48, 72, or 96 hours. The effect on germination of 1 mul/l added ethylene depended upon the amount of applied CO(2). When containers of KOH were enclosed and ambient CO(2) was absorbed, inhibition of germination by 1 mul/l exogenous ethylene was 90%. When CO(2) was applied in concentrations from 0.25 to 1.0% (v/v), CO(2) increasingly antagonized the inhibitory action of 1 mul/l added ethylene. Thus, photoinduced germination of spores was regulated by competitively interacting levels of CO(2) and ethylene.
对凤尾蕨属植物孢子萌发的调控进行了研究，方法是单独或结合应用二氧化碳和乙烯。无菌孢子在Knops 溶液中无菌播种，在松盖培养管中，单独封入 2 升室中，并在连续白光下生长。当用含有乙烯吸收剂过氯酸汞的密闭容器和富含 2%二氧化碳（v/v）的气氛保持时，孢子萌发没有任何抑制作用。较高水平的应用二氧化碳则逐渐具有抑制作用。二氧化碳的抑制作用是可逆的。当二氧化碳逸出，孢子随后暴露在环境实验室空气中时，抑制作用在 48 小时内恢复。此外，二氧化碳的抑制作用是特异性的，因为无论孢子用外源二氧化碳处理 48、72 还是 96 小时，都会产生相同程度的抑制。1 μl/L 添加乙烯对萌发的影响取决于应用二氧化碳的量。当封闭含 KOH 的容器并吸收环境中的二氧化碳时，1 μl/L 外源乙烯对萌发的抑制作用为 90%。当应用 0.25%至 1.0%（v/v）浓度的二氧化碳时，二氧化碳逐渐拮抗 1 μl/L 添加乙烯的抑制作用。因此，光诱导孢子萌发受到二氧化碳和乙烯相互竞争水平的调节。
