Institute of Natural and Environmental Resources, University of New Hampshire, Durham, New Hampshire 03824.
Plant Physiol. 1973 Nov;52(5):472-4. doi: 10.1104/pp.52.5.472.
A chamber was designed to apply up to 20 bars pressure to roots of intact plants. The unique features of this chamber are a split top arrangement to permit enclosing roots of intact plants within the chamber, a circulation coil to control temperature of rooting media, and a valve arrangement to permit changing solution without disturbing the plant. Changes in transpiration in response to changes in the pressure applied to roots of intact pepper plants illustrate one use of the equipment. Well watered plants at low light (0.05 langley/min) were observed to exude water from the leaf margins when 5 bars pressure was applied to the roots. When roots were cut off, a 1 bar pressure caused exudation. Plants with cooled roots or plants in dry soil did not exude water when as much as 6 bars pressure was applied. Transient response of transpiration rates to sudden application and release of pressure was observed in pepper and bean plants but not in rhododendron. The magnitude of this transient response was highly dependent upon light intensity and CO(2) concentration in the aerial environment.
设计了一个室，可对完整植株的根部施加高达 20 巴的压力。该室的独特之处在于采用了可拆分的顶部设计，便于将完整植株的根部封闭在室内，配置了循环线圈以控制生根介质的温度，还配置了阀门组件，可在不干扰植物的情况下更换溶液。利用该设备可观察到完整辣椒植株根部所受压力变化引起的蒸腾变化，这是设备的一种用途。在低光照（0.05 兰利/分钟）下浇水充足的植株，当根部施加 5 巴压力时，叶片边缘会有水渗出。当根部被切断时，施加 1 巴压力就会导致渗出。当施加高达 6 巴的压力时，冷却了根部的植株或处于干燥土壤中的植株都不会渗出水分。在辣椒和豆类植物中观察到蒸腾速率对压力突然施加和释放的瞬态响应，但在杜鹃属植物中则没有观察到。这种瞬态响应的幅度高度依赖于空中环境中的光照强度和 CO2 浓度。
