蓝藻异形胞中氧化和还原磷酸戊糖途径的酶活性。
Activities of enzymes of the oxidative and the reductive pentose phosphate pathways in heterocysts of a blue-green alga.
机构信息
Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823.
出版信息
Plant Physiol. 1973 Nov;52(5):480-3. doi: 10.1104/pp.52.5.480.
Abstract
Preparations of heterocysts of Anabaena cylindrica Lemm. had 7- to 8-fold higher activities of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, 2-fold more hexokinase activity, and 0.02 to 0.06 times as much ribulose diphosphate carboxylase and glyceraldehyde 3-phosphate dehydrogenase activities as did whole filaments per milligram soluble protein in cell-free extracts. Time courses of solubilization of glucose 6-phosphate dehydrogenase activity indicated that heterocysts contain 74 to 80% of the total activity of this enzyme in filaments.
摘要
柱孢鱼腥藻（Anabaena cylindrica Lemm.）异形胞的粗提物中，葡萄糖 6-磷酸脱氢酶和 6-磷酸葡萄糖酸脱氢酶的活性比完整藻丝高出 7-8 倍，己糖激酶的活性高出 2 倍，而核酮糖 1,5-二磷酸羧化酶和甘油醛-3-磷酸脱氢酶的活性则分别只有完整藻丝的 0.02-0.06 倍。无细胞抽提物中，每毫克可溶性蛋白中葡萄糖 6-磷酸脱氢酶的活性呈现出可溶的时间进程，表明异形胞中含有 74-80%的总酶活性。
相似文献
1
Activities of enzymes of the oxidative and the reductive pentose phosphate pathways in heterocysts of a blue-green alga.蓝藻异形胞中氧化和还原磷酸戊糖途径的酶活性。
Plant Physiol. 1973 Nov;52(5):480-3. doi: 10.1104/pp.52.5.480.
2
The influence of inorganic nitrogen supply on carbohydrate and related metabolism in the blue-green alga, Anabaena cylindrica Lemm.无机氮供应对蓝藻鱼腥藻属圆柱状细胞中碳水化合物及相关代谢的影响。
Planta. 1974 Sep;116(3):197-206. doi: 10.1007/BF00390227.
3
The pentose phosphate pathway of glucose metabolism. Hormonal and dietary control of the oxidative and non-oxidative reactions of the cycle in liver.葡萄糖代谢的磷酸戊糖途径。肝脏中该循环氧化反应和非氧化反应的激素及饮食调控。
Biochem J. 1969 Mar;111(5):713-25. doi: 10.1042/bj1110713.
4
The pentose phosphate pathway of glucose metabolism. Hormonal and dietary control of the oxidative nd non-oxidative reactions and related enzymes of the cycle in adipose tissue.葡萄糖代谢的磷酸戊糖途径。脂肪组织中该循环的氧化和非氧化反应及相关酶的激素和饮食调控。
Biochem J. 1969 Sep;114(2):253-64. doi: 10.1042/bj1140253.
5
Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by ribulose 1,5-diphosphate and NADPH/NADP+ ratios.菠菜叶绿体中1,5-二磷酸核酮糖以及NADPH/NADP⁺ 比值对葡萄糖-6-磷酸脱氢酶的调控
Biochim Biophys Acta. 1975 Aug 11;396(2):260-75. doi: 10.1016/0005-2728(75)90040-7.
6
The pentose phosphate pathway of glucose metabolism. Enzyme profiles and transient and steady-state content of intermediates of alternative pathways of glucose metabolism in Krebs ascites cells.葡萄糖代谢的磷酸戊糖途径。克雷布斯腹水癌细胞中葡萄糖代谢替代途径的酶谱以及中间产物的瞬态和稳态含量。
Biochem J. 1969 Dec;115(5):1009-29. doi: 10.1042/bj1151009.
7
Polyhedral bodies and ribulose 1,5-diphosphate carboxylase of the blue-green alga Anabaena cylindrica.多面体和蓝藻鱼腥藻的核酮糖 1,5-二磷酸羧化酶。
Planta. 1976 Jan;130(3):323-6. doi: 10.1007/BF00387840.
8
14C-labeled metabolites in heterocysts and vegetative cells of Anabaena cylindrica filaments and their presumptive function as transport vehicles of organic carbon and nitrogen.圆柱鱼腥藻丝状体异形胞和营养细胞中的14C标记代谢物及其作为有机碳和氮运输载体的推测功能。
J Bacteriol. 1983 Aug;155(2):628-33. doi: 10.1128/jb.155.2.628-633.1983.
9
[Enzyme formation in rye seedlings during the change from heterotrophic to autotrophic growth].[黑麦幼苗从异养生长转变为自养生长过程中的酶形成]
Planta. 1966 Dec;71(4):326-55. doi: 10.1007/BF00396320.
10
The metabolism of glucose by heterocysts and vegetative cells of Anabaena cylindrica.圆柱鱼腥藻异形胞和营养细胞对葡萄糖的代谢
Arch Microbiol. 1974;101(2):161-7. doi: 10.1007/BF00455936.
引用本文的文献
1
Genomes of nitrogen-fixing eukaryotes reveal an alternate path for organellogenesis.固氮真核生物的基因组揭示了一条细胞器发生的替代途径。
Proc Natl Acad Sci U S A. 2025 Aug 19;122(33):e2507237122. doi: 10.1073/pnas.2507237122. Epub 2025 Aug 12.
2
The endosymbiont of is specialized for nitrogen fixation within a photosynthetic eukaryote.的内共生体专门用于在光合真核生物体内进行固氮作用。
ISME Commun. 2024 Apr 15;4(1):ycae055. doi: 10.1093/ismeco/ycae055. eCollection 2024 Jan.
3
Expanding the FurC (PerR) regulon in Anabaena (Nostoc) sp. PCC 7120: Genome-wide identification of novel direct targets uncovers FurC participation in central carbon metabolism regulation.扩展鱼腥藻（念珠藻）PCC 7120 中的 FurC（PerR）调控基因簇：全基因组鉴定新的直接靶标揭示 FurC 参与中心碳代谢调控。
PLoS One. 2023 Aug 7;18(8):e0289761. doi: 10.1371/journal.pone.0289761. eCollection 2023.
4
Mechanisms for Generating Low Potential Electrons across the Metabolic Diversity of Nitrogen-Fixing Bacteria.固氮细菌代谢多样性产生低势能电子的机制。
Appl Environ Microbiol. 2023 May 31;89(5):e0037823. doi: 10.1128/aem.00378-23. Epub 2023 May 8.
5
Reconstruction of a regulated two-cell metabolic model to study biohydrogen production in a diazotrophic cyanobacterium Anabaena variabilis ATCC 29413.构建调控两细胞代谢模型研究固氮蓝藻鱼腥藻 ATCC 29413 生物制氢
PLoS One. 2020 Jan 24;15(1):e0227977. doi: 10.1371/journal.pone.0227977. eCollection 2020.
6
Metalloproteins in the Biology of Heterocysts.异形胞生物学中的金属蛋白。
Life (Basel). 2019 Apr 3;9(2):32. doi: 10.3390/life9020032.
7
Optimization of carbon dioxide and nitrogen fixation by the blue-green alga Anabaena in freshwater blooms.淡水水华中蓝藻鱼腥藻对二氧化碳和氮固定的优化
Oecologia. 1979 Jan;38(3):275-290. doi: 10.1007/BF00345188.
8
Analysis of the early heterocyst Cys-proteome in the multicellular cyanobacterium Nostoc punctiforme reveals novel insights into the division of labor within diazotrophic filaments.对多细胞蓝细菌点状念珠藻早期异形胞半胱氨酸蛋白质组的分析揭示了对固氮丝状体中分工的新见解。
BMC Genomics. 2014 Dec 4;15(1):1064. doi: 10.1186/1471-2164-15-1064.
9
Ribulose 1,5-bisphosphate carboxylase and polyhedral bodies of Chlorogloeopsis fritschii.核酮糖-1,5-二磷酸羧化酶和 Chlorogloeopsis fritschii 的多面体。
Planta. 1981 Nov;153(3):279-85. doi: 10.1007/BF00383900.
本文引用的文献
1
Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.固氮蓝藻的研究。I. 柱状鱼腥藻的生长与固氮作用
Plant Physiol. 1955 Jul;30(4):366-72. doi: 10.1104/pp.30.4.366.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Dark and photometabolism of sugars by a blue green alga: Tolypothrix tenuis.一种蓝绿藻——纤细席藻对糖的暗代谢和光代谢
Plant Physiol. 1966 Apr;41(4):731-7. doi: 10.1104/pp.41.4.731.
4
Incorporation of amino sugars into walls during heterocyst differentiation.在异形胞分化过程中氨基糖掺入细胞壁。
Dev Biol. 1971 Sep;26(1):159-64. doi: 10.1016/0012-1606(71)90115-1.
5
Is the heterocyst the site of nitrogen fixation in blue-green algae?异形胞是蓝藻中固氮作用的场所吗？
Nature. 1968 Nov 23;220(5169):810-2. doi: 10.1038/220810b0.
6
Tetrazolium reduction and nitrogenase activity in heterocystous blue-green algae.异形胞蓝藻中的四氮唑还原作用和固氮酶活性
Arch Mikrobiol. 1972;87(4):341-52. doi: 10.1007/BF00409133.
7
Metabolism of glucose by unicellular blue-green algae.单细胞蓝藻对葡萄糖的代谢
Arch Mikrobiol. 1972;87(4):303-22. doi: 10.1007/BF00409131.
8
Photosynthetic and dark carbon metabolism in unicellular blue-green algae.单细胞蓝藻中的光合与暗碳代谢
Arch Mikrobiol. 1972;86(1):25-38. doi: 10.1007/BF00412397.
9
Absence of photosystem 2 in heterocysts of the blue-green alga Anabaena.蓝藻鱼腥藻异形胞中光系统2的缺失。
Biochim Biophys Acta. 1972 Jan 21;256(1):157-61. doi: 10.1016/0005-2728(72)90170-3.
10
The incorporation and metabolism of glucose by Anabaena variabilis.多变鱼腥藻对葡萄糖的摄取与代谢
J Gen Microbiol. 1968 Dec;54(3):451-62. doi: 10.1099/00221287-54-3-451.
Zotero 插件