Department of Biochemistry and Division of Natural Sciences, North Dakota State University, Fargo, North Dakota 58102.
Plant Physiol. 1974 Jan;53(1):1-4. doi: 10.1104/pp.53.1.1.
Differential centrifugation of several plant extracts indicates that the majority of the hydroperoxide isomerase activity is present in the cytoplasm of the cell. However, lesser amounts of isomerase activity were found in the mitochondrial and microsomal fractions of sunflower seedlings. Sucrose density gradient centrifugation of extracts from sunflower, watermelon, and flax seedlings and from cauliflower buds showed that isomerase activity was associated with the mitochondria. There was no evidence for presence of hydroperoxide isomerase activity in the microbodies.
几种植物提取物的差速离心表明，大多数过氧化物异构酶活性存在于细胞的细胞质中。然而，在向日葵幼苗的线粒体和微粒体部分也发现了较少的异构酶活性。从向日葵、西瓜和亚麻幼苗以及花椰菜芽中提取的物质进行蔗糖密度梯度离心表明，异构酶活性与线粒体有关。没有证据表明微体中存在过氧化物异构酶活性。
