Department of Biology, Brandeis University, Waltham, Massachusetts 02154.
Plant Physiol. 1974 Feb;53(2):140-3. doi: 10.1104/pp.53.2.140.
Factors affecting CO(2) fixation in the spinach (Spinacia oleracea) chloroplast were investigated. Free magnesium ions are shown to be highly inhibitory for photosynthetic CO(2) fixation in isolated intact spinach chloroplasts. The pH optimum for CO(2) fixation is about 8.5 but is dependent upon the reaction medium. Conditions are defined under which chloroplasts illuminated in the absence of CO(2) accumulate ribulose 1,5-diphosphate, and fix CO(2) in a subsequent dark period when high magnesium ion concentrations are provided. The regulation of photosynthetic CO(2) assimilation by these factors is discussed.
研究了影响菠菜(Spinacia oleracea)叶绿体中 CO2 固定的因素。实验表明,游离镁离子对分离完整的菠菜叶绿体的光合 CO2 固定有高度抑制作用。CO2 固定的最适 pH 值约为 8.5,但取决于反应介质。在一定条件下,当提供高浓度镁离子时,叶绿体在无 CO2 的情况下被照光,会积累核酮糖 1,5-二磷酸,并在随后的黑暗期固定 CO2。讨论了这些因素对光合 CO2 同化的调节。