Miginiac-Maslow M, Champigny M L
Physiologie Cellulaire Végétale, Centre National de la Recherche Scientifique, Université Paris-Sud, 91405 Orsay, France.
Plant Physiol. 1974 Jun;53(6):856-62. doi: 10.1104/pp.53.6.856.
The changes in the levels of intact spinach (Spinacia oleracea L.) chloroplast adenine nucleotides during the time course of light-dependent CO(2) fixation were determined with respect to the effect of antimycin A. This study demonstrated that antimycin A lowered the rate of ATP formation during the induction period of carboxylation. While the steady state levels of ATP and the energy-charge value also decreased in the presence of antimycin, the concomitant increase of the CO(2) fixation activities insured higher ATP turnover rates. Changes in the labeling of CO(2) fixation products during the lag phase suggested a stepwise activation of the Calvin cycle, with fructose 1,6-diphosphate, and ribulose 5-phosphate kinase being activated before ribulose 1,5-diphosphate carboxylase. The possible mechanisms of the enhancement of CO(2) fixation activity by antimycin A in relation to its action on photophosphorylation during the lag phase are discussed.
就抗霉素A的作用而言，测定了完整菠菜（Spinacia oleracea L.）叶绿体腺嘌呤核苷酸水平在光依赖型CO₂固定过程中的变化。本研究表明，抗霉素A降低了羧化诱导期ATP的形成速率。虽然在抗霉素存在下ATP的稳态水平和能量电荷值也降低，但CO₂固定活性的同时增加确保了更高的ATP周转率。延迟期CO₂固定产物标记的变化表明卡尔文循环是逐步激活的，1,6-二磷酸果糖和5-磷酸核酮糖激酶在1,5-二磷酸核酮糖羧化酶之前被激活。讨论了抗霉素A在延迟期增强CO₂固定活性与其对光合磷酸化作用相关的可能机制。
