Borchert R, McChesney J D, Watson D
Department of Botany, University of Kansas, Lawrence, Kansas 66044.
Plant Physiol. 1974 Feb;53(2):187-91. doi: 10.1104/pp.53.2.187.
Several aspects of wound healing in tuber tissue of potato (Solanum tuberosum var. Kennebec), known to require protein synthesis, are inhibited by 2,4-dichlorobenzyltributylphosphonium chloride (Phosphon D). Cell division was completely blocked by 60 mum Phosphon and markedly reduced by concentrations as low as 3 mum. When applied at the time of wounding, 0.25mm Phosphon completely prevented the wound-induced respiratory increase. Application at 15 hours after wounding arrested respiration at the rate present at that time. The same concentrations of Phosphon inhibited auxin-induced cell expansion of the tissue, protein synthesis as measured by the incorporation of leucine-(14)C into the trichloroacetic acid-insoluble fraction of tissue disks, and the appearance of wound-induced peroxidase isozymes. None of these inhibitory effects of Phosphon could be prevented or reversed by the application of gibberellic acid. All wound-induced processes inhibited by Phosphon are also inhibited by cycloheximide. It is suggested that inhibitory effects of Phosphon on wound healing in potato and on other developmental processes in excised plant tissues which cannot be reversed by gibberellin are due to interference with protein synthesis.
已知马铃薯（Solanum tuberosum var. Kennebec）块茎组织的伤口愈合需要蛋白质合成，其多个方面受到2,4 - 二氯苄基三丁基氯化鏻（Phosphon D）的抑制。60 μmol的Phosphon可完全阻断细胞分裂，低至3 μmol的浓度也能显著降低细胞分裂。在受伤时施加0.25 mmol的Phosphon可完全阻止伤口诱导的呼吸增加。在受伤后15小时施加则使呼吸停止在当时的速率。相同浓度的Phosphon抑制组织的生长素诱导的细胞扩张、通过将亮氨酸 -（14）C掺入组织圆盘的三氯乙酸不溶部分所测量的蛋白质合成，以及伤口诱导的过氧化物酶同工酶的出现。Phosphon的这些抑制作用均不能通过施加赤霉素来预防或逆转。Phosphon抑制的所有伤口诱导过程也受到环己酰亚胺的抑制。有人认为，Phosphon对马铃薯伤口愈合以及对不能被赤霉素逆转的离体植物组织中其他发育过程的抑制作用是由于对蛋白质合成的干扰。
