Division of Biological Sciences, Cornell University, Ithaca, New York 14850.
Plant Physiol. 1974 Feb;53(2):220-3. doi: 10.1104/pp.53.2.220.
The bicarbonate effect in stimulating the rate of photophosphorylation by isolated spinach (Spinacia oleracea var. Virginia blight-resistant savoy) chloroplasts at a pH below the optimum has been re-examined. Its seasonal nature may be related to the hormonal status of the plants. Bicarbonate anions stimulate adenosine 5'-triphosphate synthesis if added in the final, adenosine 5'-triphosphate-forming stage of either a postillumination or an acid-base experiment. They also stimulate the membrane-bound, Mg(2+)-dependent adenosine 5'-triphosphatase of chloroplasts, and the Ca(2+)-dependent adenosine 5'-triphosphatase of detached coupling factor. These and other data point to the interaction between energized thylakoid membranes and the coupling factor as the probable site of action of bicarbonate anions when they stimulate photophosphorylation.
在 pH 值低于最佳值的情况下，用分离的菠菜（Spinacia oleracea var. Virginia blight-resistant savoy）叶绿体刺激光磷酸化速率的碳酸氢盐效应已被重新研究。其季节性特征可能与植物的激素状态有关。如果在光照后或酸碱实验的最后一个，即生成三磷酸腺苷的阶段添加碳酸氢根阴离子，它们可以刺激三磷酸腺苷的合成。它们还刺激叶绿体结合的、Mg(2+)依赖的三磷酸腺苷酶和分离的偶联因子的 Ca(2+)依赖的三磷酸腺苷酶。这些和其他数据表明，当碳酸氢根阴离子刺激光磷酸化时，它们与被激活的类囊体膜和偶联因子之间的相互作用可能是其作用的可能部位。
