Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1974 Feb;53(2):317-8. doi: 10.1104/pp.53.2.317.
A high proportion of the soluble peroxidase of tobacco (Nicotiana tabacum L. var. Bottom Special) leaves is found in the fluid obtained by centrifugation of a buffer solution previously infiltrated into the intercellular spaces. Only a very small amount of the cytoplasmic enzyme, glucose 6-phosphate dehydrogenase, is present in this fluid. Specific activity data suggest that an active process is responsible for the transfer of soluble peroxidase to the intercellular space and that the intercellular fluid fraction is not simply composed of material moving out of leaf cells by diffusion. The centrifugation method is a satisfactory means of isolating diluted intercellular fluid for biochemical and physiological investigations.
烟草（Nicotiana tabacum L. var. Bottom Special）叶片中高比例的可溶过氧化物酶存在于先前渗透到细胞间隙的缓冲溶液经离心获得的液体中。只有非常少量的细胞质酶，葡萄糖 6-磷酸脱氢酶，存在于这种液体中。活性数据表明，一种活跃的过程负责将可溶过氧化物酶转移到细胞间隙，并且细胞间隙液部分不是简单地由通过扩散离开叶细胞的物质组成。离心法是一种用于生化和生理研究的分离稀释细胞间隙液的满意方法。
