玉米胚乳酸性蛋白酶的特性。
Characteristics of an Acid protease from maize endosperm.
机构信息
Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1.
出版信息
Plant Physiol. 1974 Mar;53(3):449-52. doi: 10.1104/pp.53.3.449.
An assay has been developed to measure protease activity in endosperm extracts of maize seeds. With hemoglobin as substrate, the enzyme(s) has a pH optimum of 3.8 and a temperature optimum of 46 C. It also degrades gliadin, edestin, bovine serum albumin, and partially hydrolyzed zein and glutelin under standard assay conditions. The enzyme(s) has endopeptidase activity with all substrates tested. When undenatured zein and glutelin are suspended in an agar gel, both are efficiently degraded. Using this assay, the protease activity increases from day 3 to day 8 after inhibition and then declines.
已开发出一种测定玉米种子胚乳提取物中蛋白酶活性的方法。以血红蛋白为底物，该酶的 pH 最适值为 3.8，温度最适值为 46°C。在标准测定条件下，它还能降解麦醇溶蛋白、醇溶谷蛋白、牛血清白蛋白和部分水解的玉米醇溶蛋白和谷蛋白。该酶对所有测试的底物均具有内肽酶活性。当天然玉米醇溶蛋白和谷蛋白悬浮在琼脂凝胶中时，两者都能被有效降解。使用该测定法，蛋白酶活性在抑制后第 3 天至第 8 天增加，然后下降。
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