Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1.
Plant Physiol. 1974 Mar;53(3):453-7. doi: 10.1104/pp.53.3.453.
Degradation of the major storage proteins in maize endosperm, zein and glutelin, begins during the 2nd day of germination. The protein most abundant in the mature endosperm is degraded most rapidly. The patterns of protein loss are essentially similar in germinating seeds and excised endosperms. Cycloheximide, added at the beginning of the incubation period, prevents the development of alpha-amylase and protease activities and the disappearance of starch and protein reserves. Late additions (70 hours) of cycloheximide still inhibit the increase in hydrolase activity but have no effect on the hydrolysis of storage reserves. The results indicate that the hydrolytic enzymes are synthesized de novo in the maize endosperm.
玉米胚乳中主要贮藏蛋白（醇溶蛋白和谷蛋白）的降解始于发芽的第 2 天。成熟胚乳中含量最丰富的蛋白质降解最快。在萌发的种子和离体胚乳中，蛋白质的丧失模式基本相似。在孵育期开始时添加环己亚胺可防止α-淀粉酶和蛋白酶活性的发展以及淀粉和蛋白质储备的消失。环己亚胺的后期添加（70 小时）仍然抑制水解酶活性的增加，但对贮藏物的水解没有影响。结果表明，水解酶是在玉米胚乳中从头合成的。
