Department of Plant Sciences, University of London King's College, 68 Half Moon Lane, London SE24 9JF England.
Plant Physiol. 1974 May;53(5):691-3. doi: 10.1104/pp.53.5.691.
At concentrations of up to 300 mug/ml both d-threo- and l-threo-chloramphenicol act as energy transfer inhibitors in spinach chloroplasts, in that they inhibit both phosphorylation and phosphorylating electron transport, without affecting the nonphosphorylating electron transport which occurs either in the absence of a phosphate acceptor or in the presence of the uncoupler ammonium chloride. At higher concentrations, there appears to be an additional site of chloramphenicol inhibition of electron transport. If d-threo-chloramphenicol is to be used as a protein synthesis inhibitor in intact chloroplasts or tissues, control experiments with another chloramphenicol isomer seem to be necessary.
在高达 300 微克/毫升的浓度下，d-苏式和 l-苏式氯霉素都可作为菠菜叶绿体中的能量转移抑制剂，因为它们既抑制磷酸化作用又抑制磷酸化电子传递，而不影响非磷酸化电子传递，后者发生在没有磷酸受体的情况下或在解偶联剂氯化铵存在的情况下。在较高的浓度下，氯霉素对电子传递的抑制似乎还有另外一个部位。如果 d-苏式氯霉素要在完整的叶绿体或组织中用作蛋白质合成抑制剂，那么似乎需要用另一种氯霉素异构体进行对照实验。
